MethPrimer Input Form

Paste an ORIGINAL source sequence. Try this Sample sequence You don't need to modify your sequence (e.g. convert 'C' to 'T') before pasting.

	Pick primers for bisulfite sequencing PCR or restriction PCR .
	Pick MSP primers.

Use CpG island prediction for primer selection?

Window

Shift

Obs/Exp

GC%

General Parameters for Primer Selection
Sequence name (optional):
Target (optional):	"start, size", such as (560, 30)
Excluded Regions (optional):	"start, size", such as (160, 50 1100, 50)
Number of output pairs (optional):

Product Size:	Min:	Opt:	Max:
Primer Tm:	Min:	Opt:	Max:
Primer Size:	Min:	Opt:	Max:
Product CpGs:		Primer Poly X:
Primer non-CpG 'C's:		Primer Poly T:

Parameters for MSP primers
3'CpG constraint:
CpG in primer:
Max Tm difference:

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How to cite MethPrimer: Li LC and Dahiya R. MethPrimer: designing primers for methylation PCRs. Bioinformatics. 2002 Nov;18(11):1427-31. PMID: 12424112