MethPrimer
Design primers for bisulfite-converted DNA with an easy, research-ready workflow.
Trusted by researchers since 2002
Bisulfite PCR
Generate primers for bisulfite sequencing or restriction PCR with CpG-aware rules.
MSP Primers
Design methylated vs. unmethylated primer sets to support MSP experiments.
CpG Islands
Optionally scan CpG islands and target regions for primer selection.
How It Works
1. Paste Sequence
Provide your DNA sequence in any standard format.
2. Set Parameters
Adjust CpG island settings, primer sizes, and product constraints.
3. Get Results
Review primer pairs and predicted product details instantly.
How to Cite MethPrimer
Li LC, Dahiya R. MethPrimer: designing primers for methylation PCRs. Bioinformatics. 2002 Nov;18(11):1427-31.